Prophage, phiPV83-pro, carrying panton-valentine leukocidin genes, on the Staphylococcus aureus P83 chromosome: comparative analysis of the genome structures of phiPV83-pro, phiPVL, phi11, and other phages

Biosci Biotechnol Biochem. 2000 Dec;64(12):2631-43. doi: 10.1271/bbb.64.2631.

Abstract

Staphylococcus aureus P83 has Panton-Valentine leukocidin (PVL)-like genes, lukM and lukF-PV. Here, lukM and lukF-PV genes were found on the genome of a prophage, which was designated as phiPV83-pro. The precise genome size was 45,636 bp with att core sequences of 10 base pairs. Sixty-four ORFs were identified on the phiPV83-pro genome, including two extra operons, lukM-lukF-PV and orfs63-64. The lukM-lukF-PV cluster was located 2.1 kb upstream of the attL site. The most striking feature of the phiPV83-pro genome was a constituent of at least 4 regions from phi11, phiPVL, and other phages, i.e., (i) att sites identical with those of phi11, (ii) a cos sequence and the genes encoding packaging and head proteins of phiPVL (occupied half region of phiPV83-pro), and (iii) the other two regions which showed no significant similarity with known phages (occupied about 40% of phiPV83-pro). Furthermore, two insertion sequences, ISSA1 and ISSA2 were integrated into attL site and orf44, respectively. PhiPV83-pro was not induced as phage particles from S. aureus P83 regardless of its treatment with mitomycin C. The insertion of ISSA1 into the attL site was one of the reasons of the failure of the induction of the phage particles by mitomycin C treatment of the strain P83.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / genetics
  • Bacterial Toxins
  • Base Sequence
  • Chromosomes, Bacterial*
  • DNA Replication / genetics
  • DNA Transposable Elements
  • Exotoxins
  • Gene Order
  • Genome, Viral*
  • Leukocidins / genetics*
  • Molecular Sequence Data
  • Multigene Family
  • Sequence Analysis, DNA
  • Sequence Homology, Nucleic Acid
  • Staphylococcus aureus / genetics*
  • Viral Proteins / genetics
  • Virus Activation / genetics
  • Virus Replication / genetics

Substances

  • Bacterial Proteins
  • Bacterial Toxins
  • DNA Transposable Elements
  • Exotoxins
  • Leukocidins
  • LukM protein, Staphylococcus aureus
  • Panton-Valentine leukocidin
  • Viral Proteins
  • LukF protein, Staphylococcus aureus

Associated data

  • GENBANK/AB010124
  • GENBANK/AB044554