The localization of persistent foot and mouth disease virus in the epithelial cells of the soft palate and pharynx

J Comp Pathol. Feb-Apr 2001;124(2-3):89-94. doi: 10.1053/jcpa.2000.0431.


After contact with foot and mouth disease virus (FMDV), cattle may become persistently infected, regardless of their pre-existing immune status or whether they develop clinical disease. The cellular sites of FMDV persistence have not previously been determined. The use of in-situ hybridization in combination with tyramide signal amplification (TSA) provided the first direct evidence that FMDV RNA is localized within the epithelial cells of the soft palate and pharynx during persistent infection, indicating that these cells remain persistently infected after contact with FMDV.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Aphthovirus / genetics
  • Aphthovirus / isolation & purification*
  • Body Fluids / virology
  • Cattle
  • Cattle Diseases / pathology
  • Cattle Diseases / virology*
  • Epithelial Cells / pathology
  • Epithelial Cells / virology*
  • Foot-and-Mouth Disease / pathology
  • Foot-and-Mouth Disease / virology*
  • In Situ Hybridization / veterinary
  • Oligonucleotide Probes / chemistry
  • Palate, Soft / pathology
  • Palate, Soft / virology*
  • Pharynx / pathology
  • Pharynx / virology*
  • RNA, Viral / analysis


  • Oligonucleotide Probes
  • RNA, Viral