Phase-variable expression of an operon encoding extracellular alkaline protease, a serine protease homolog, and lipase in Pseudomonas brassicacearum

J Bacteriol. 2001 Mar;183(6):2117-20. doi: 10.1128/JB.183.6.2117-2120.2001.

Abstract

The rhizobacterium Pseudomonas brassicacearum forms phenotypic variants which do not show extracellular protease and lipase activity. The operon encoding these enzymes, a serine protease homolog, and a type I secretion machinery was characterized. Transcriptional lacZ gene fusions revealed that the expression of the operon is under the control of phase variation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP-Binding Cassette Transporters*
  • Bacterial Proteins / chemistry
  • Bacterial Proteins / genetics*
  • Bacterial Proteins / metabolism
  • Cosmids
  • Escherichia coli / enzymology
  • Escherichia coli / genetics
  • Gene Expression Regulation, Bacterial
  • Lipase / chemistry
  • Lipase / genetics*
  • Lipase / metabolism
  • Membrane Proteins*
  • Membrane Transport Proteins*
  • Molecular Sequence Data
  • Operon*
  • Promoter Regions, Genetic
  • Pseudomonas / enzymology
  • Pseudomonas / genetics*
  • Pseudomonas / physiology
  • Recombinant Proteins / metabolism
  • Sequence Analysis, DNA
  • Subtilisins / chemistry
  • Subtilisins / genetics*
  • Subtilisins / metabolism

Substances

  • ATP-Binding Cassette Transporters
  • AprD protein, Pseudomonas fluorescens
  • AprE protein, Bacteria
  • AprF protein, Pseudomonas
  • Bacterial Proteins
  • LipA protein, Bacteria
  • Membrane Proteins
  • Membrane Transport Proteins
  • Recombinant Proteins
  • Lipase
  • Subtilisins

Associated data

  • GENBANK/AF286062