A clinically approved oral vaccine against pneumotropic bacteria induces the terminal maturation of CD83+ immunostimulatory dendritic cells

Immunol Lett. 2001 Feb 1;76(1):63-7. doi: 10.1016/s0165-2478(00)00326-6.

Abstract

Dendritic cells (DCs) are important antigen-presenting cells of the immune system that have attracted interest as cellular adjuvants to induce immunity in clinical settings. We have investigated the effects of Broncho-Vaxom, an oral vaccine composed of lysates from eight pneumotropic bacteria, on human monocyte-derived dendritic cells (moDCs). Broncho-Vaxom induced the terminal maturation of CD83+ moDCs. MoDCs stimulated with Broncho-Vaxom displayed a phenotype of activated DCs with high levels of major histocompatibility complex (MHC) molecules and increased levels of adhesion and co-stimulatory molecules. In addition, moDCs activated with Broncho-Vaxom exhibited enhanced T cell-stimulatory capacity in the allogeneic mixed leukocyte reaction. Broncho-Vaxom at 100 microg/ml was as potent as TNF-alpha at 1000 U/ml in activating human moDCs. Neither LPS-like activity nor bacterial DNA was found to be responsible for the maturation-inducing activity of Broncho-Vaxom, suggesting that Broncho-Vaxom contains other bacterial factors that are capable of inducing the terminal maturation of moDCs. In DC-based immunotherapy, Broncho-Vaxom could be used as a stimulus of DC maturation, which meets the standards of good manufacturing practice (GMP). In addition, vaccination with Broncho-Vaxom-loaded moDCs may be an attractive treatment option in preventing recurrent airway infection in predisposed individuals.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adjuvants, Immunologic / administration & dosage
  • Administration, Oral
  • Antigens, CD
  • Bacteria*
  • Bacterial Vaccines / administration & dosage*
  • Bacterial Vaccines / immunology*
  • Cell Differentiation / immunology
  • Cell Extracts*
  • Cells, Cultured
  • Dendritic Cells / cytology*
  • Dendritic Cells / immunology*
  • Humans
  • Immunoglobulins / immunology*
  • Immunophenotyping
  • Interferon Inducers / immunology
  • Lymphocyte Activation / immunology*
  • Membrane Glycoproteins / immunology*
  • Monocytes / cytology
  • Monocytes / immunology
  • Pneumonia, Bacterial / immunology*
  • Pneumonia, Bacterial / prevention & control
  • T-Lymphocytes / immunology

Substances

  • Adjuvants, Immunologic
  • Antigens, CD
  • Bacterial Vaccines
  • Broncho-Vaxom
  • CD83 antigen
  • Cell Extracts
  • Immunoglobulins
  • Interferon Inducers
  • Membrane Glycoproteins