Intracerebroventricularly administered corticotropin-releasing factor inhibits food intake and produces anxiety-like behaviour at very low doses in mice

Diabetes Obes Metab. 1999 Sep;1(5):281-4. doi: 10.1046/j.1463-1326.1999.00033.x.

Abstract

Aim: Previous studies have demonstrated that corticotropin-releasing factor (CRF) produces behavioural, physiological and immunological responses similar to those induced by stress. However, these findings have been validated largely in laboratory rats.

Methods: We examined the effects of intracerebroventricular (i.c.v.) administration of CRF on anxiety and food intake in mice. Using the elevated-plus maze, we measured anxiety levels after i.c.v. CRF in mice. We also measured food intake for 2 h after i.c.v. CRF.

Results: CRF increased the normal preference for the closed arms of the maze at a very low dose of 3 pmol, indicating an anxiogenic effect. CRF powerfully suppressed food intake at the doses of 3-300 pmol for over 2 h.

Conclusion: Our results demonstrate that i.c.v. CRF evokes anxiogenic behaviour and suppresses feeding with the same dose-response relationships in mice. CRF may thus play a role in integrating the overall responses to stress through co-ordinated actions in the brain of this species.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anxiety / chemically induced*
  • Behavior, Animal / drug effects*
  • Corticotropin-Releasing Hormone / administration & dosage*
  • Corticotropin-Releasing Hormone / pharmacology
  • Eating / drug effects*
  • Injections, Intraventricular
  • Kinetics
  • Male
  • Mice
  • Motor Activity / drug effects

Substances

  • Corticotropin-Releasing Hormone