Catalytic activities of membrane-type 6 matrix metalloproteinase (MMP25)

FEBS Lett. 2001 Feb 23;491(1-2):137-42. doi: 10.1016/s0014-5793(01)02150-0.


This study describes the biochemical characterisation of the catalytic domain of membrane-type 6 matrix metalloproteinase (MT6-MMP, MMP25, leukolysin). Its activity towards synthetic peptide substrates, components of the extracellular matrix and inhibitors of MMPs was studied and compared with MT1-MMP, MT4-MMP and stromelysin-1. We have found that MT6-MMP is closer in function to stromelysin-1 than MT1 and MT4-MMP in terms of substrate and inhibitor specificity, being able to cleave type-IV collagen, gelatin, fibronectin and fibrin. However, it differs from stromelysin-1 and MT1-MMP in its inability to cleave laminin-I, and unlike stromelysin-1 cannot activate progelatinase B. Our findings suggest that MT6-MMP could play a role in cellular migration and invasion of the extracellular matrix and basement membranes and its activity may be tightly regulated by all members of the TIMP family.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal
  • Blotting, Western
  • Catalytic Domain
  • Electrophoresis, Polyacrylamide Gel
  • Extracellular Matrix / enzymology
  • Extracellular Matrix / metabolism*
  • GPI-Linked Proteins
  • Humans
  • Hydrolysis
  • Matrix Metalloproteinase 3 / chemistry
  • Matrix Metalloproteinase 3 / metabolism
  • Matrix Metalloproteinase Inhibitors
  • Matrix Metalloproteinases / chemistry
  • Matrix Metalloproteinases / metabolism*
  • Matrix Metalloproteinases, Membrane-Associated
  • Metalloendopeptidases / chemistry
  • Metalloendopeptidases / metabolism
  • Protein Folding
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / metabolism
  • Substrate Specificity


  • Antibodies, Monoclonal
  • GPI-Linked Proteins
  • Matrix Metalloproteinase Inhibitors
  • Recombinant Proteins
  • MMP17 protein, human
  • Matrix Metalloproteinases
  • Matrix Metalloproteinases, Membrane-Associated
  • Metalloendopeptidases
  • matrix metalloproteinase 25
  • Matrix Metalloproteinase 3