Activation of antigen-specific T cells by artificial cell constructs having immobilized multimeric peptide-class I complexes and recombinant B7-Fc proteins

J Immunol Methods. 2001 Mar 1;249(1-2):111-9. doi: 10.1016/s0022-1759(00)00335-5.

Abstract

T cell activation results from the engagement of multiple receptors on T cells by their respective ligands on antigen presenting cells. Studies using artificial cell surface constructs have demonstrated that effective T cell response requires that antigen be presented on a solid surface with dimensions that approximate those of an intact cell. In this report, we describe the cloning and expression of recombinant B7-1-Fc and B7-2-Fc proteins and their incorporation onto 5-microm latex microspheres along with renatured peptide-MHC. These microspheres provide a simple and effective method for the in vitro or in vivo stimulation of antigen-specific T cells under precisely controlled antigen and costimulation conditions.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antigen Presentation*
  • B7-1 Antigen / genetics
  • B7-1 Antigen / immunology*
  • Cells, Immobilized / immunology
  • Histocompatibility Antigens Class I / immunology*
  • Immunoglobulin Fc Fragments / genetics
  • Immunoglobulin Fc Fragments / immunology*
  • Immunologic Techniques
  • Lymphocyte Activation*
  • Mice
  • Mice, Transgenic
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / immunology
  • T-Lymphocytes / immunology*

Substances

  • B7-1 Antigen
  • Histocompatibility Antigens Class I
  • Immunoglobulin Fc Fragments
  • Recombinant Fusion Proteins