Extracellular enzyme system utilized by the fungus Sporotrichum pulverulentum (Chrysosporium lignorum) for the breakdown of cellulose. 2. Activities of the five endo-1,4-beta-glucanases towards carboxymethylcellulose

Eur J Biochem. 1975 Feb 3;51(1):207-11. doi: 10.1111/j.1432-1033.1975.tb03920.x.

Abstract

A complementary discussion is given of an earlier presented theory for viscometric determination of enzymic activity towards polymer substrates. Five different, purified endo-1,4-beta-glucanases from the fungus Sporotrichum pulverulentum have been characterised with respect to their molecular activities(Am) and Michaelis-Menten constants (Km). The errors in the Am and Km values are discussed. The Am value seems to be more valuable as a distinguishing parameter than the Km value.

MeSH terms

  • Carboxymethylcellulose Sodium / metabolism*
  • Cellulase / metabolism*
  • Glycoside Hydrolases / metabolism*
  • Kinetics
  • Mathematics
  • Methylcellulose / analogs & derivatives*
  • Sporothrix / enzymology*
  • Viscosity

Substances

  • Methylcellulose
  • Glycoside Hydrolases
  • Cellulase
  • Carboxymethylcellulose Sodium