Elevated interleukin-4 and interleukin-13 production by T cell lines from patients with Churg-Strauss syndrome

Arthritis Rheum. 2001 Feb;44(2):469-73. doi: 10.1002/1529-0131(200102)44:2<469::AID-ANR66>3.0.CO;2-0.


Objective: To investigate cytokine production patterns of T cell lines (TCL) from patients with Churg-Strauss syndrome (CSS).

Methods: Short-term polyclonal TCL were generated from peripheral blood of patients with CSS or Wegener's granulomatosis (WG) and healthy controls (HC). TCL were established in the presence of interleukin-2 (IL-2) and phytohemagglutinin and were phenotypically characterized by flow cytometry. Th1/ Th2 cytokine production by stimulated TCL (72 hours) was analyzed by enzyme-linked immunosorbent assay.

Results: TCL that represented the progeny of in vivo-activated T cells from CSS patients displayed a heterogeneous immunophenotype, with a predominance of CD4+ T cells when compared with WG TCL, which were predominantly CD8+. All CSS TCL shared the ability to produce large amounts of interferon-gamma (IFNgamma), IL-4, and IL-13 compared with HC (P = 0.014 for all 3). Production of IL-4 and IL-13 was higher in CSS TCL than in WG TCL (P = 0.014 for both). IL-5 production was up-regulated in WG TCL compared with CSS TCL (P = 0.014). Compared with HC, WG TCL showed increased production of IFNgamma (P = 0.021), IL-5 (P = 0.043), and IL-13 (P = 0.021).

Conclusion: Our results indicate that, while there is evidence for both a type 1 and a type 2 response in CSS, type 2 cytokine production pattern appears to predominate in this disease when compared with WG and HC.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Cell Line / metabolism
  • Churg-Strauss Syndrome / metabolism*
  • Churg-Strauss Syndrome / pathology
  • Cytokines / biosynthesis
  • Cytokines / blood
  • Female
  • Granulomatosis with Polyangiitis / metabolism
  • Granulomatosis with Polyangiitis / pathology
  • Humans
  • Interferon-gamma / biosynthesis
  • Interleukin-10 / biosynthesis
  • Interleukin-13 / biosynthesis*
  • Interleukin-4 / biosynthesis*
  • Interleukin-5 / biosynthesis
  • Male
  • Middle Aged
  • T-Lymphocytes / cytology*


  • Cytokines
  • Interleukin-13
  • Interleukin-5
  • Interleukin-10
  • Interleukin-4
  • Interferon-gamma