A physical map of human chromosome 14

Nature. 2001 Feb 15;409(6822):947-8. doi: 10.1038/35057177.


We report the construction of a tiling path of around 650 clones covering more than 99% of human chromosome 14. Clone overlap information to assemble the map was derived by comparing fully sequenced clones with a database of clone end sequences (sequence tag connector strategy). We selected homogeneously distributed seed points using an auxiliary high-resolution radiation hybrid map comprising 1,895 distinct positions. The high long-range continuity and low redundancy of the tiling path indicates that the sequence tag connector approach compares favourably with alternative mapping strategies.

Publication types

  • Classical Article

MeSH terms

  • Chromosomes, Artificial, Bacterial
  • Chromosomes, Human, Pair 14*
  • Cloning, Molecular
  • Escherichia coli
  • Humans
  • Physical Chromosome Mapping
  • Radiation Hybrid Mapping
  • Sequence Tagged Sites