Antiplatelet and anticancer isothiocyanates in Japanese domestic horseradish, wasabi

Biofactors. 2000;13(1-4):271-6. doi: 10.1002/biof.5520130141.


6-Methylsulfinylhexyl isothiocyanate (MS-ITC) was isolated from wasabi (Wasabia japonica, Japanese domestic horseradish) as a potential inhibitor of human platelet aggregation in vitro through our extensive screening of vegetables and fruits. In the course of another screening for the induction of glutathione S-transferase (GST) activity in RL34 cells. MS-ITC was inadvertently isolated from wasabi as a potential inducer of GST. MS-ITC administered to rats or mice also showed both activities in vivo. As a result from elucidation of the platelet aggregation inhibition and the GST induction mechanisms of MS-ITC, the isothiocyanate moiety of MS-ITC plays an important role for antiplatelet and anticancer activities because of its highly reactivity with sulfhydryl (-SH) groups in biomolecules (GSH, cysteine residue in a certain protein, etc.).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antineoplastic Agents, Phytogenic / isolation & purification
  • Antineoplastic Agents, Phytogenic / pharmacology*
  • Brassicaceae / chemistry*
  • Cell Line
  • Fruit / chemistry*
  • Glutathione Transferase / biosynthesis
  • Humans
  • Isothiocyanates / isolation & purification
  • Isothiocyanates / pharmacology*
  • Japan
  • Platelet Aggregation / drug effects
  • Platelet Aggregation Inhibitors / isolation & purification
  • Platelet Aggregation Inhibitors / pharmacology*
  • Spices / analysis*
  • Vegetables / chemistry*


  • 6-methylsulfinylhexyl isothiocyanate
  • Antineoplastic Agents, Phytogenic
  • Isothiocyanates
  • Platelet Aggregation Inhibitors
  • Glutathione Transferase