Interleukin 15 stimulates production of matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 by human peripheral blood mononuclear cells

Cytokine. 2001 Feb 21;13(4):244-7. doi: 10.1006/cyto.2000.0818.

Abstract

In peripheral blood mononuclear cells (PBMC), matrix metalloproteinase (MMP)-9 mediates the extravasation of immune cells and may be involved in tissue destruction during inflammation. We investigated the effect of the pro-inflammatory cytokines interleukin (IL-)12 and 15 on the secretion of MMP-9 in PBMC. IL-15, but not IL-12, induces MMP-9 in PBMC and in T cells. Moreover, the combination of IL-15 and IL-2 had an additive effect. In contrast, both IL-12 and IL-15 induced the release of tissue inhibitor of metalloproteinases (TIMP)-1. IL-15 led to a dose-dependent increase of the MMP-9/TIMP-1 ratio as a measure for increased proteolytic capacity. We conclude that IL-15 mediates its effects in inflammation in part through MMP-9.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cells, Cultured
  • Enzyme Activation / immunology
  • Enzyme Precursors / analysis
  • Enzyme-Linked Immunosorbent Assay
  • Humans
  • Interleukin-15 / pharmacology*
  • Leukocytes, Mononuclear / enzymology*
  • Leukocytes, Mononuclear / immunology
  • Lymphocyte Activation
  • Matrix Metalloproteinase 2 / analysis
  • Matrix Metalloproteinase 9 / analysis
  • Matrix Metalloproteinase 9 / biosynthesis*
  • T-Lymphocytes / enzymology
  • T-Lymphocytes / immunology
  • Tissue Inhibitor of Metalloproteinase-1 / analysis
  • Tissue Inhibitor of Metalloproteinase-1 / biosynthesis*

Substances

  • Enzyme Precursors
  • Interleukin-15
  • Tissue Inhibitor of Metalloproteinase-1
  • Matrix Metalloproteinase 2
  • Matrix Metalloproteinase 9