Identification of a novel type I cytokine receptor CRL2 preferentially expressed by human dendritic cells and activated monocytes

Biochem Biophys Res Commun. 2001 Mar 9;281(4):878-83. doi: 10.1006/bbrc.2001.4432.


From a human dendritic cell (DC) cDNA library, we identified a novel type I cytokine receptor, designated as cytokine receptor-like molecule 2 (CRL2). CRL2 cDNA encoded a 371-residue type I transmembrane protein with an extracellular domain of 210 residues and an intracellular domain of 119 residues. Its extracellular domain contains conserved cysteine residues and WAS-like motif in place of the hallmark of WSXWS motif present in other type I cytokine receptors. The intracellular domain contained a membrane-proximal "box 1" motif and conserved tyrosine residue potentially as a binding site for signal transducing molecules. CRL2 protein shares significant homology with common cytokine receptor (gammac) and interleukin-13 receptor alpha1 chain. Northern blot analysis showed that CRL2 was restrictedly expressed by spleen and peripheral blood leukocytes, and abundantly expressed by HL-60 cells. RT-PCR analysis demonstrated that CRL2 was preferentially expressed by DC and monocytes. Interestingly, CRL2 expression was up-regulated when monocytes were activated by LPS. These indicate that CRL2 may be involved in the biological functions of DC and monocytes. The Ba/F3 transfectants of CRL2 was retrovirally established with the expressed FLAG-tagged CRL2 in the size of approximately 48 kD, which could be efficiently immunoprecipitated. We also prepared a CRL2Ig fusion protein. The identification of its ligand and involvement of signal transduction will help to elucidate its potential function.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Northern
  • DNA, Complementary / chemistry
  • DNA, Complementary / genetics
  • DNA, Complementary / isolation & purification
  • Dendritic Cells / cytology
  • Dendritic Cells / metabolism*
  • Female
  • Gene Expression Regulation
  • HL-60 Cells
  • HeLa Cells
  • Humans
  • K562 Cells
  • Male
  • Molecular Sequence Data
  • Monocytes / cytology
  • Monocytes / metabolism*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Receptors, Cytokine / genetics*
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Tissue Distribution
  • Tumor Cells, Cultured
  • U937 Cells


  • CRLF2 protein, human
  • DNA, Complementary
  • RNA, Messenger
  • Receptors, Cytokine

Associated data

  • GENBANK/AF142570