Duplex ultrasound (DU) is recognised as a valuable tool for the assessment of blood flow in many vascular territories. The application of this technique to the superior mesenteric artery (SMA) has increased rapidly throughout the last decade. The purpose of this review is to collate currently available information on the utility of SMA DU, both in terms of research and clinical practice. Research investigations have revealed low intra- and interobserver variability in the estimation of Doppler variables, while reliable evaluation of B-mode dimensions requires repeated measurements. SMA blood flow velocity has been found to be dependent upon changes in central haemodynamics and in peripheral resistance, which was documented in studies with hypotension, medication and post-prandially. Food intake induces mesenteric vasorelaxation reflected by a 10-fold increase in the diastolic velocity. This feature has been utilised in studies on mesenteric physiology, which confirmed parasympathetic activity during hypovolaemia, and showed that exercise increases splanchnic resistance and reduces its blood flow following a 50% reduction in the hepato-splenic and a 25% reduction in the mesenteric blood flow. Clinical studies have documented high sensitivity and specificity of DU in detection of disease in splanchnic arteries. Diastolic velocity was found to be the most accurate indicator of SMA stenosis, while an absent Doppler signal from a well visualised vessel has been found to be a reliable predictor of occlusion. The high predictive value of DU in the detection of mesenteric artery disease, together with its simplicity and non-invasiveness, suggests that DU should take precedence over arteriography in both clinical practice and laboratory investigations.