Protein kinase D regulates the fission of cell surface destined transport carriers from the trans-Golgi network

Cell. 2001 Feb 9;104(3):409-20. doi: 10.1016/s0092-8674(01)00228-8.


When a kinase inactive form of Protein Kinase D (PKD-K618N) was expressed in HeLa cells, it localized to the trans-Golgi network (TGN) and caused extensive tubulation. Cargo that was destined for the plasma membrane was found in PKD-K618N-containing tubes but the tubes did not detach from the TGN. As a result, the transfer of cargo from TGN to the plasma membrane was inhibited. We have also demonstrated the formation and subsequent detachment of cargo-containing tubes from the TGN in cells stably expressing low levels of PKD-K618N. Our results suggest that PKD regulates the fission from the TGN of transport carriers that are en route to the cell surface.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Biological Transport
  • CD4 Antigens / metabolism
  • Cell Line
  • Cell Membrane / enzymology*
  • Cell Membrane / metabolism
  • Clathrin / metabolism
  • Coatomer Protein / metabolism
  • Dose-Response Relationship, Drug
  • Endoplasmic Reticulum / metabolism
  • Endosomes / metabolism
  • Fluorescent Antibody Technique
  • Glutathione Transferase / metabolism
  • Glycoproteins*
  • HeLa Cells
  • Humans
  • Membrane Glycoproteins / metabolism
  • Membrane Proteins*
  • Phosphorylation
  • Precipitin Tests
  • Protein Kinase C / genetics
  • Protein Kinase C / physiology*
  • Protein Transport
  • Recombinant Proteins / metabolism
  • Temperature
  • Time Factors
  • Transfection
  • Viral Envelope Proteins / metabolism
  • trans-Golgi Network / metabolism*


  • CD4 Antigens
  • Clathrin
  • Coatomer Protein
  • G protein, vesicular stomatitis virus
  • Glycoproteins
  • Membrane Glycoproteins
  • Membrane Proteins
  • Recombinant Proteins
  • TGOLN2 protein, human
  • Viral Envelope Proteins
  • Glutathione Transferase
  • protein kinase D
  • Protein Kinase C