Interleukin 1beta, interleukin-6, and tumor necrosis factor-alpha in endometriotic tissue and in endometrium

Fertil Steril. 2001 Mar;75(3):489-95. doi: 10.1016/s0015-0282(00)01752-0.

Abstract

Objective: To compare the levels of IL-1beta, IL-6, and TNFalpha in endometriotic tissue and in endometrium from women with endometriosis and healthy controls.

Design: Open.

Setting: Department of Obstetrics and Gynecology at a university hospital.

Patient(s): Twenty-six women with endometriosis and 22 controls operated on for clinical indications.

Intervention(s): ELISA in homogenized tissue samples collected during surgery.

Main outcome measure(s): Levels of IL-1beta, IL-6, and TNFalpha in tissue homogenates.

Result(s): The three types of tissue differed significantly with respect to all three cytokines. Endometriotic tissue had significantly higher concentrations of IL-1beta than endometrium from both patients with endometriosis and healthy controls. Both endometriotic tissue and endometrium from patients had significantly higher concentrations of IL-6, and endometriotic tissue had significantly lower concentration of TNFalpha than did endometrium from controls. IL-1beta showed a cycle phase dependence that was significant in endometrium from patients, being higher in the secretory than in the follicular phase. IL-1beta was significantly higher in endometrioma than in lesions of other localizations. Concentrations of IL-1beta and IL-6 were positively correlated in endometriotic tissue and in endometrium from controls. No other significant correlations were found.

Conclusion(s): This study has shown a significant production of IL-1beta, IL-6, and TNFalpha in endometriotic tissue and endometrium, with significant differences between the tissue types, indicating a deviating cytokine pattern in both endometriotic tissue and endometrium from women with endometriosis compared with that from healthy controls.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Endometriosis / metabolism*
  • Endometrium / chemistry*
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Humans
  • Interleukin-1 / analysis*
  • Interleukin-6 / analysis*
  • Menstrual Cycle
  • Ovarian Diseases / metabolism
  • Tumor Necrosis Factor-alpha / analysis*

Substances

  • Interleukin-1
  • Interleukin-6
  • Tumor Necrosis Factor-alpha