doi: 10.1006/meth.2000.1131.
A simple and efficient method to transcribe RNAs with reduced 3' heterogeneity
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- PMID: 11243833
- DOI: 10.1006/meth.2000.1131
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A simple and efficient method to transcribe RNAs with reduced 3' heterogeneity
Methods.
2001 Mar.
Abstract
Ribose C2' methoxy groups (-OCH3) on the penultimate nucleotide or the last two nucleotides of DNA templates can dramatically improve the quality of transcripts produced by T7 RNA polymerase. This strategy can be adapted to generate transcripts of varying lengths and will allow greater ease in purification of the products.
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