Identification of Lhcb1/Lhcb2/Lhcb3 heterotrimers of the main light-harvesting chlorophyll a/b-protein complex of Photosystem II (LHC II)

Biochim Biophys Acta. 2001 Apr 2;1504(2-3):340-5. doi: 10.1016/s0005-2728(00)00262-0.

Abstract

Using non-denaturing isoelectric focusing in polyacrylamide vertical slab gel, we have purified to homogeneity three trimeric subcomplexes of LHC II from Arabidopsis thylakoid membranes. The polypeptide composition of the subcomplexes were studied by immunoblotting. Our results indicate the existence in vivo of LHC II heterotrimers containing Lhcb1, Lhcb2 and Lhcb3 gene products.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoproteins / chemistry
  • Arabidopsis / chemistry*
  • Arabidopsis Proteins / chemistry*
  • Circular Dichroism
  • Isoelectric Focusing
  • Light-Harvesting Protein Complexes / chemistry*
  • Peptides / chemistry
  • Photosynthetic Reaction Center Complex Proteins / chemistry*
  • Photosystem II Protein Complex
  • Thylakoids / chemistry

Substances

  • Apoproteins
  • Arabidopsis Proteins
  • Lhcb2 protein, Arabidopsis
  • Light-Harvesting Protein Complexes
  • Peptides
  • Photosynthetic Reaction Center Complex Proteins
  • Photosystem II Protein Complex