Cholinergic neurons expressing neuromedin K receptor (NK3) in the basal forebrain of the rat: a double immunofluorescence study

Neuroscience. 2001;103(2):413-22. doi: 10.1016/s0306-4522(00)00568-6.

Abstract

By using a double immunofluorescence method we have examined the distribution of cholinergic neurons expressing neuromedin K receptor (NK3) in the rat brain and spinal cord. The distribution of neuromedin K receptor-like immunoreactive neurons completely overlapped with that of choline acetyltransferase-positive neurons in certain regions of the basal forebrain, e.g. the medial septal nucleus, nucleus of the diagonal band of Broca, magnocellular preoptic nucleus and substantia innominata. Partially overlapping distributions of neuromedin K receptor-like immunoreactive and choline acetyltransferase-positive neurons were found in the basal nucleus of Meynert, globus pallidus, ventral pallidum of the forebrain, tegmental nuclei of the pons and dorsal motor nucleus of the vagus. Neurons showing both neuromedin K receptor-like and choline acetyltransferase immunoreactivities, however, were found predominantly in the medial septal nucleus, nucleus of the diagonal band of Broca and magnocellular preoptic nucleus of the basal forebrain: 66-80% of these choline acetyltransferase-positive neurons displayed neuromedin K receptor-like immunoreactivity. Neurons showing both neuromedin K receptor-like and choline acetyltransferase immunoreactivities were hardly detected in other aforementioned regions of the forebrain, brainstem and spinal cord. The present study has provided morphological evidence for direct physiological modulation or regulation of cholinergic neurons by tachykinins through the neuromedin K receptor in the basal forebrain of rats.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Basal Nucleus of Meynert / chemistry
  • Basal Nucleus of Meynert / cytology
  • Choline O-Acetyltransferase / analysis
  • Cholinergic Fibers / chemistry*
  • Cholinergic Fibers / enzymology
  • Corpus Striatum / chemistry
  • Corpus Striatum / cytology
  • Cranial Nerves / chemistry
  • Cranial Nerves / cytology
  • Diagonal Band of Broca / chemistry
  • Diagonal Band of Broca / cytology
  • Fluorescent Antibody Technique
  • Male
  • Motor Neurons / chemistry
  • Motor Neurons / enzymology
  • Pons / chemistry
  • Pons / cytology
  • Preoptic Area / chemistry
  • Preoptic Area / cytology
  • Rats
  • Rats, Sprague-Dawley
  • Receptors, Neurokinin-3 / analysis*
  • Receptors, Neurokinin-3 / biosynthesis
  • Septal Nuclei / chemistry*
  • Septal Nuclei / cytology
  • Spinal Cord / chemistry
  • Spinal Cord / cytology
  • Tegmentum Mesencephali / chemistry
  • Tegmentum Mesencephali / cytology

Substances

  • Receptors, Neurokinin-3
  • Choline O-Acetyltransferase