Use of functional proteomics to investigate PKC epsilon-mediated cardioprotection: the signaling module hypothesis

Am J Physiol Heart Circ Physiol. 2001 Apr;280(4):H1434-41. doi: 10.1152/ajpheart.2001.280.4.H1434.


The characterization of biological processes on the basis of alterations in the cellular proteins, or "proteomic" analysis, is a powerful approach that may be adopted to decipher the signaling mechanisms that underlie various pathophysiological conditions, such as ischemic heart disease. This review represents a prospectus for the implementation of proteomic analyses to delineate the myocardial intracellular signaling events that evoke cardioprotection against ischemic injury. In concert with this, the manifestation of a protective phenotype has recently been shown to involve dynamic modulation of protein kinase C-epsilon (PKC epsilon) signaling complexes (Ping P, Zhang J, Pierce WM Jr, and Bolli R. Circ Res 88: 59--62, 2001). Accordingly, "the signaling module hypothesis" is formulated as a plausible mechanism by which multipurpose stress-activated proteins and signaling kinases may function collectively to facilitate the genesis of cardioprotection.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Animals
  • Cardiotonic Agents*
  • Heart / physiology*
  • Heart / physiopathology
  • Humans
  • Isoenzymes / metabolism*
  • Models, Cardiovascular*
  • Myocardial Ischemia / physiopathology
  • Protein Kinase C / metabolism*
  • Protein Kinase C-epsilon
  • Proteome / metabolism*
  • Signal Transduction / physiology*


  • Cardiotonic Agents
  • Isoenzymes
  • Proteome
  • PRKCE protein, human
  • Protein Kinase C
  • Protein Kinase C-epsilon