Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 268 (6), 1596-604

Biosynthesis of Cannabinoids. Incorporation Experiments With (13)C-labeled Glucoses

Affiliations

Biosynthesis of Cannabinoids. Incorporation Experiments With (13)C-labeled Glucoses

M Fellermeier et al. Eur J Biochem.

Abstract

The biosynthesis of cannabinoids was studied in cut sprouts of Cannabis sativa by incorporation experiments using mixtures of unlabeled glucose and [1-(13)C]glucose or [U-(13)C(6)]glucose. (13)C-labeling patterns of cannabichromenic acid and tetrahydrocannabinolic acid were analyzed by quantitative NMR spectroscopy. (13)C enrichments and coupling patterns show that the C(10)-terpenoid moiety is biosynthesized entirely or predominantly (> 98%) via the recently discovered deoxyxylulose phosphate pathway. The phenolic moiety is generated by a polyketide-type reaction sequence. The data support geranyl diphosphate and the polyketide, olivetolic acid, as specific intermediates in the biosynthesis of cannabinoids.

Similar articles

See all similar articles

Cited by 23 PubMed Central articles

See all "Cited by" articles

Publication types

MeSH terms

LinkOut - more resources

Feedback