BLIMP-1: trigger for differentiation of myeloid lineage

Nat Immunol. 2000 Aug;1(2):169-76. doi: 10.1038/77861.

Abstract

B lymphocyte-induced maturation protein-1 (BLIMP-1 or PRDI-BF1) is induced when bone marrow-derived progenitors differentiate in response to macrophage-colony stimulating factor (M-CSF) and is present in peripheral blood monocytes and granulocytes. BLIMP-1 is also induced during differentiation of U937 and HL-60 cells into macrophages or granulocytes. Induction of BLIMP-1 mRNA during macrophage differentiation of U937 and HL-60 shows a biphasic pattern. Overexpression of BLIMP-1 is sufficient to initiate macrophage differentiation of U937 cells whereas blocking endogenous BLIMP-1 inhibits differentiation. One target of BLIMP-1-dependent transcriptional repression in U937 cells is c-myc, providing an explanation for cessation of cell division. Thus BLIMP-1 is a key regulator of terminal differentiation in two separate hematopoietic lineages: myeloid cells and B lymphocytes.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cell Differentiation*
  • Cell Lineage
  • Gene Expression
  • HL-60 Cells
  • Humans
  • Positive Regulatory Domain I-Binding Factor 1
  • Proto-Oncogene Proteins c-myc / genetics
  • Repressor Proteins*
  • Transcription Factors / biosynthesis
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*
  • U937 Cells
  • Zinc Fingers*

Substances

  • Proto-Oncogene Proteins c-myc
  • Repressor Proteins
  • Transcription Factors
  • PRDM1 protein, human
  • Positive Regulatory Domain I-Binding Factor 1