Molecular Retrieval of Large 16S rRNA Gene Fragments From an Italian Rice Paddy Soil Affiliated With the Class Actinobacteria

Syst Appl Microbiol. 2000 Dec;23(4):582-4. doi: 10.1016/S0723-2020(00)80033-8.

Abstract

We designed a PCR assay specific for the 16S rRNA genes of members of the class Actinobacteria, and created a clone library using the amplification product of total community DNA extracted from anoxic Italian rice field soil. Eighteen out of 27 randomly sequenced clones were affiliated with Actinobacteria, i.e. Frankineae, Corynebacterineae, Micrococcineae, the bacterium candidatus "Microthrix parvicella" and a novel taxonomically undefined cluster.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actinobacteria / classification
  • Actinobacteria / genetics
  • Actinobacteria / isolation & purification*
  • Cloning, Molecular
  • DNA, Ribosomal / genetics
  • DNA, Ribosomal / isolation & purification*
  • Evolution, Molecular
  • Genes, rRNA
  • Italy
  • Molecular Sequence Data
  • Oryza / microbiology*
  • Polymerase Chain Reaction / methods
  • RNA, Ribosomal, 16S / genetics*
  • Soil Microbiology*

Substances

  • DNA, Ribosomal
  • RNA, Ribosomal, 16S

Associated data

  • GENBANK/AJ277687
  • GENBANK/AJ277688
  • GENBANK/AJ277689
  • GENBANK/AJ277690
  • GENBANK/AJ277691
  • GENBANK/AJ277692
  • GENBANK/AJ277693
  • GENBANK/AJ277694
  • GENBANK/AJ277695
  • GENBANK/AJ277696
  • GENBANK/AJ277697
  • GENBANK/AJ277698
  • GENBANK/AJ277699
  • GENBANK/AJ277700
  • GENBANK/AJ277701
  • GENBANK/AJ277836
  • GENBANK/AJ277837
  • GENBANK/AJ277838