The ins and outs of nucleosome assembly

Curr Opin Genet Dev. 2001 Apr;11(2):136-41. doi: 10.1016/s0959-437x(00)00170-2.


De novo nucleosome assembly coupled to DNA replication and repair in vitro involves the histone chaperone chromatin assembly factor 1 (CAF-1). Recent studies support a model in which CAF-1 can be targeted to newly synthesized DNA through a direct interaction with proliferating cell nuclear antigen (PCNA) and can act synergistically with a newly identified histone chaperone. Insights have also been obtained into mechanisms by which this CAF-1-dependent pathway can establish a repressed chromatin state.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Acetylation
  • Animals
  • Chromatin Assembly Factor-1
  • Chromosomal Proteins, Non-Histone*
  • DNA / metabolism*
  • DNA Repair
  • DNA Replication*
  • DNA-Binding Proteins / metabolism
  • Histones / metabolism
  • Models, Biological
  • Molecular Chaperones / metabolism
  • Nucleosomes / chemistry
  • Nucleosomes / metabolism*
  • Proliferating Cell Nuclear Antigen / metabolism


  • Chromatin Assembly Factor-1
  • Chromosomal Proteins, Non-Histone
  • DNA-Binding Proteins
  • Histones
  • Molecular Chaperones
  • Nucleosomes
  • Proliferating Cell Nuclear Antigen
  • DNA