Gene expression profiling of malignant mesothelioma cell lines: cDNA array study

Int J Cancer. 2001 Feb 15;91(4):492-6. doi: 10.1002/1097-0215(200002)9999:9999<::aid-ijc1094>;2-m.


To reveal genes relevant for malignant mesothelioma (MM), we carried out cDNA array experiments on 4 MM cell lines and 2 primary mesothelial cell cultures established from pleural fluid of non-cancer patients. Human cancer gene filters including 588 genes were used for the cDNA array experiments. Our study revealed 26 over-expressed genes that play a role in the regulation of cell fate, cell cycle, cell growth and DNA damage repair and 13 under-expressed genes encoding growth factors, receptors and proteins involved in cell adhesion, motility and invasion to be common to 3 or 4 MM cell lines. We confirmed the cDNA array results using RT-PCR for 5 of the over-expressed genes and for 3 of the under-expressed genes. Our study presents gene expression profiles in MM cell lines and shows the involvement of several genes, such as those encoding JAGGED1, ser/thr protein kinase NIK, Ku80 and cyclin D2, novel in MM.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA, Complementary / metabolism*
  • Down-Regulation
  • Electrophoresis, Agar Gel
  • Epithelium / metabolism
  • Gene Expression Regulation, Neoplastic*
  • Humans
  • Mesothelioma / genetics*
  • Mesothelioma / metabolism*
  • Nucleic Acid Hybridization
  • Oligonucleotide Array Sequence Analysis*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Tumor Cells, Cultured
  • Up-Regulation


  • DNA, Complementary