Post-transcriptional gene silencing by double-stranded RNA

Nat Rev Genet. 2001 Feb;2(2):110-9. doi: 10.1038/35052556.


Imagine being able to knock out your favourite gene with only a day's work. Not just in one model system, but in virtually any organism: plants, flies, mice or cultured cells. This sort of experimental dream might one day become reality as we learn to harness the power of RNA interference, the process by which double-stranded RNA induces the silencing of homologous endogenous genes. How this phenomenon works is slowly becoming clear, and might help us to develop an effortless tool to probe gene function in cells and animals.

Publication types

  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Animals
  • Gene Silencing*
  • Models, Biological
  • RNA Processing, Post-Transcriptional*
  • RNA, Double-Stranded / metabolism*


  • RNA, Double-Stranded