CCR6-deficient mice have impaired leukocyte homeostasis and altered contact hypersensitivity and delayed-type hypersensitivity responses

J Clin Invest. 2001 Mar;107(6):R37-45. doi: 10.1172/JCI11297.


CCR6 expression in dendritic, T, and B cells suggests that this beta-chemokine receptor may regulate the migration and recruitment of antigen-presenting and immunocompetent cells during inflammatory and immunological responses. Here we demonstrate that CCR6-/- mice have underdeveloped Peyer's patches, in which the myeloid CD11b+ CD11c+ dendritic-cell subset is not present in the subepithelial dome. CCR6-/- mice also have increased numbers in T-cell subpopulations within the intestinal mucosa. In 2,4-dinitrofluorobenzene-induced contact hypersensitivity (CHS) studies, CCR6-/- mice developed more severe and more persistent inflammation than wild-type (WT) animals. Conversely, in a delayed-type hypersensitivity (DTH) model induced with allogeneic splenocytes, CCR6-/- mice developed no inflammatory response. The altered responses seen in the CHS and DTH assays suggest the existence of a defect in the activation and/or migration of the CD4(+) T-cell subsets that downregulate or elicit the inflammation response, respectively. These findings underscore the role of CCR6 in cutaneous and intestinal immunity and the utility of CCR6-/- mice as a model to study pathologies in these tissues. This article was published online in advance of the print edition. The date of publication is available from the JCI website,

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CD4-Positive T-Lymphocytes / immunology
  • Cell Movement
  • Dermatitis, Contact / genetics*
  • Dermatitis, Contact / immunology*
  • Female
  • Homeostasis
  • Hypersensitivity, Delayed / genetics*
  • Hypersensitivity, Delayed / immunology*
  • Intestinal Mucosa / immunology
  • Intestinal Mucosa / pathology
  • Langerhans Cells / immunology
  • Leukocytes / immunology*
  • Leukocytes / pathology
  • Lymphocyte Activation
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Peyer's Patches / immunology
  • Peyer's Patches / pathology
  • Receptors, CCR6
  • Receptors, Chemokine / deficiency*
  • Receptors, Chemokine / genetics
  • Receptors, Chemokine / physiology


  • CCR6 protein, human
  • Receptors, CCR6
  • Receptors, Chemokine