DNA motif associated with meiotic double-strand break regions in Saccharomyces cerevisiae

EMBO Rep. 2000 Sep;1(3):232-8. doi: 10.1093/embo-reports/kvd047.


Meiotic recombination in yeast is initiated by DNA double-strand breaks (DSBs) that occur at preferred sites, distributed along the chromosomes. These DSB sites undergo changes in chromatin structure early in meiosis, but their common features at the level of DNA sequence have not been defined until now. Alignment of 1 kb sequences flanking six well-mapped DSBs has allowed us to define a flexible sequence motif, the CoHR profile, which predicts the great majority of meiotic DSB locations. The 50 bp profile contains a poly(A) tract in its centre and may have several gaps of unrelated sequences over a total length of up to 250 bp. The major exceptions to the correlation between CoHRs and preferred DSB sites are at telomeric regions, where DSBs do not occur. The CoHR sequence may provide the basis for understanding meiosis-induced chromatin changes that enable DSBs to occur at defined chromosomal sites.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Argininosuccinate Lyase
  • Base Sequence
  • Chromatin / genetics
  • Chromatin / metabolism
  • Chromosome Breakage / genetics*
  • Chromosomes, Fungal / genetics
  • DNA, Fungal / chemistry
  • DNA, Fungal / genetics*
  • DNA, Fungal / metabolism
  • Fungal Proteins / genetics
  • Genes, Fungal / genetics
  • Meiosis / genetics*
  • Nucleic Acid Conformation
  • Promoter Regions, Genetic / genetics
  • Recombination, Genetic / genetics*
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae Proteins*
  • Sequence Alignment
  • Sequence Homology, Nucleic Acid


  • Chromatin
  • DNA, Fungal
  • Fungal Proteins
  • Saccharomyces cerevisiae Proteins
  • Arg4 protein, S cerevisiae
  • Argininosuccinate Lyase