Kinetics of apoptosis-related genes mRNA expression in the dorsal skin of hypotrichotic WBN/ILA-ht rats after topical application of T-2 toxin

Exp Toxicol Pathol. 2001 Feb;52(6):553-6. doi: 10.1016/s0940-2993(01)80016-6.


The expression of apoptosis-related genes mRNAs was examined in the dorsal skin of hypotrichotic WBN/ILA-Ht rats topically applied with T-2 toxin (10 microl of 0.5 microg/microl solution). The total mRNA was obtained from skin biopsy samples from each rat at 3, 6, 12 and 24 hours after T-2 toxin treatment (HAT), and RT-PCR was carried out with pairs of oligonucleotide primers corresponding to the cDNA sequences of rat p53, bcl-2, c-ki-ras, c-fos and c-jun oncogenes. The expression of c-fos mRNA markedly increased at 3 HAT, peaked at 6 HAT, and greatly decreased at 12 HAT. However it maintained a higher level, compared with the control level, even at 24 HAT. Although not prominent, the expression of c-jun mRNA also showed significant elevation from 3 to 12 HAT. On the other hand, there were no changes in the expression of p53, bcl-2 and c-ki-ras mRNAs throughout the observation period. Judging from the present results and our previous report that epidermal cells developed apoptosis at 12 HAT (Histol Histopathol 1999; 14: 337-342), the induction of c-fos and perhaps of c-jun mRNAs may be associated with T-2 toxin-induced epidermal cell apoptosis.

MeSH terms

  • Animals
  • Apoptosis / genetics*
  • DNA Primers / chemistry
  • Genes, bcl-2 / genetics
  • Genes, fos / genetics
  • Genes, jun / genetics
  • Genes, p53 / genetics
  • Genes, ras / genetics
  • Hypotrichosis* / genetics
  • Male
  • RNA, Messenger / biosynthesis*
  • Rats
  • Rats, Wistar
  • Reverse Transcriptase Polymerase Chain Reaction
  • Skin / drug effects*
  • Skin / metabolism
  • Skin / pathology
  • T-2 Toxin / toxicity*
  • Time Factors


  • DNA Primers
  • RNA, Messenger
  • T-2 Toxin