Restricted growth of ent(-) and tonB mutants of Salmonella enterica serovar Typhi in human Mono Mac 6 monocytic cells

FEMS Microbiol Lett. 2001 Mar 1;196(1):7-11. doi: 10.1111/j.1574-6968.2001.tb10532.x.

Abstract

Monocytes and macrophages are an important host defense in humans infected with Salmonella enterica serovar Typhi. Bacterial ability to survive in these cells is therefore a crucial virulence characteristic of this pathogen. In this study, we demonstrate that growth of a Salmonella enterica serovar Typhi enterochelin synthesis mutant and a tonB mutant in the human monocyte cell line Mono Mac 6 is restricted compared to that of the parental wild-type Ty2 strain. These results suggest that enterochelin- and TonB-mediated iron uptake plays a role in S. enterica serovar Typhi pathogenesis, and also suggest that mutations in iron uptake may attenuate S. enterica serovar Typhi strains for human beings.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism*
  • Biological Transport
  • Cell Line
  • DNA Transposable Elements
  • Enterobactin / metabolism*
  • Genes, Bacterial
  • Humans
  • Iron / metabolism
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism*
  • Monocytes / microbiology*
  • Mutation
  • Salmonella typhi / genetics*
  • Salmonella typhi / growth & development*
  • Salmonella typhi / pathogenicity
  • Virulence

Substances

  • Bacterial Proteins
  • DNA Transposable Elements
  • Membrane Proteins
  • tonB protein, Bacteria
  • Enterobactin
  • Iron