Excretion of radioactive diadzein and equol as monosulfates and disulfates in the urine of the laying hen

Can J Biochem. 1975 Feb;53(2):223-30. doi: 10.1139/o75-031.


The phytoestrogen, diadzein, was injected intramuscularly as [4-14-C]daidzein into two laying hens. The radioactive materials in the urine for the succeeding 23 (hen 1) or 14 (hen 2) days were fractionated on a DEAE-Sephadex column by a gradient of NaCl and the fractions thus separated were further analyzed by solvent partition, susceptability to enzymic cleavage and thin-layer chromatography. The sic following components were identified and quantitated: [14-C]diadzein, [14-C]equol, [14-C]diadzein monosulfate, [14-C]equol monosulfate, [14-C]diazein disulfate, and [14-C]equol disulfate. The urine from hen 2 yielded also the sulfate of an unidentified conversion product of [14-C]daidzein. Repeared tests for glucuronides of [14-C]daidzein or its conversion products gave negative results, excluding the possibility that any appreciable proportion of the radioactivity in the urine was in the form of beta-glucuronide. It is concluded that the diadzein and the equol excretion in the urine of the laying hen are present for the most part as monosulfates and disulfates.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Chickens
  • Chromatography, Ion Exchange
  • Chromatography, Thin Layer
  • Crystallization
  • Estradiol Congeners / urine*
  • Female
  • Glucuronates / urine
  • Ovulation*
  • Sulfuric Acids / urine*
  • Time Factors


  • Estradiol Congeners
  • Glucuronates
  • Sulfuric Acids