Solution structure of the DNA-binding domain of TraM

Biochemistry. 2001 Mar 20;40(11):3370-7. doi: 10.1021/bi002031c.

Abstract

The solution structure of the DNA-binding domain of the TraM protein, an essential component of the DNA transfer machinery of the conjugative resistance plasmid R1, is presented. The structure has been determined using homonuclear 2-dimensional NMR spectroscopy as well as 15N labeled heteronuclear 2- and 3-dimensional NMR spectroscopy. It turns out that the solution structure of the DNA binding domain of the TraM protein is globular and dominantly helical. The very first amino acids of the N-terminus are unstructured.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / chemistry*
  • Bacterial Proteins / genetics
  • Circular Dichroism
  • DNA-Binding Proteins / chemistry*
  • DNA-Binding Proteins / genetics
  • Escherichia coli / chemistry
  • Escherichia coli / genetics
  • Nuclear Magnetic Resonance, Biomolecular / methods
  • Peptide Fragments / chemistry
  • Peptide Fragments / genetics
  • Plasmids / chemistry
  • Protein Conformation
  • Protein Structure, Secondary / genetics
  • Protein Structure, Tertiary / genetics
  • Recombinant Proteins / chemistry
  • Solutions
  • Thermodynamics

Substances

  • Bacterial Proteins
  • DNA-Binding Proteins
  • Peptide Fragments
  • Recombinant Proteins
  • Solutions
  • TraM protein, bacterial

Associated data

  • PDB/1DP3