A MAGE-3 peptide recognized on HLA-B35 and HLA-A1 by cytolytic T lymphocytes

Tissue Antigens. 2001 Feb;57(2):103-9. doi: 10.1034/j.1399-0039.2001.057002103.x.


Antigens encoded by MAGE genes are of particular interest for cancer immunotherapy because of their strict tumoral specificity and because they are shared by many tumors. Antigenic peptide EVDPIGHLY encoded by MAGE-3 and known to be presented by HLA-A*0101 is currently being used in therapeutic vaccination trials. We report here that a cytolytic T-lymphocyte (CTL) clone, which is restricted by HLA-B*3501, recognizes the same peptide and, importantly, lyses HLA-B*3501 tumor cells expressing MAGE-3. These results infer that the current clinical use of peptide EVDPIGHLY can now be extended to HLA-B*3501 patients.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Antigens, Neoplasm*
  • CD8-Positive T-Lymphocytes / immunology
  • CD8-Positive T-Lymphocytes / metabolism
  • Dendritic Cells / immunology
  • Dendritic Cells / metabolism
  • Epitopes / immunology
  • Gene Expression / immunology
  • HLA-A1 Antigen / immunology*
  • HLA-B35 Antigen / immunology*
  • Humans
  • Interferon-gamma / metabolism
  • Melanoma
  • Molecular Sequence Data
  • Neoplasm Proteins / genetics
  • Neoplasm Proteins / immunology*
  • T-Lymphocytes, Cytotoxic / immunology*
  • T-Lymphocytes, Cytotoxic / metabolism
  • Transfection
  • Tumor Cells, Cultured


  • Antigens, Neoplasm
  • Epitopes
  • HLA-A1 Antigen
  • HLA-B35 Antigen
  • MAGEA3 protein, human
  • Neoplasm Proteins
  • Interferon-gamma