Boron neutron capture therapy of a murine mammary carcinoma using a lipophilic carboranyltetraphenylporphyrin

Radiat Res. 2001 Apr;155(4):603-10. doi: 10.1667/0033-7587(2001)155[0603:bnctoa]2.0.co;2.

Abstract

The first control of a malignant tumor in vivo by porphyrin- mediated boron neutron capture therapy (BNCT) is described. In mice bearing implanted EMT-6 mammary carcinomas, boron uptake using a single injection of either p-boronophenylalanine (BPA) or mercaptoundecahydrododecaborane (BSH) was compared with either a single injection or multiple injections of the carboranylporphyrin CuTCPH. The BSH and BPA doses used were comparable to the highest doses of these compounds previously administered in a single injection to rodents. For BNCT, boron concentrations averaged 85 microg (10)B/g in the tumor and 4 microg (10)B/g in blood 2 days after the last of six injections (over 32 h) that delivered a total of 190 microg CuTCPH/g body weight. During a single 15, 20, 25 or 30 MW-min exposure to the thermalized neutron beam of the Brookhaven Medical Research Reactor, a tumor received average absorbed doses of approximately 39, 52, 66 or 79 Gy, respectively. A long-term (>200 days) tumor control rate of 71% was achieved at a dose of 66 Gy with minimal damage to the leg. Equivalent long-term tumor control by a single exposure to 42 Gy X rays was achieved, but with greater damage to the irradiated leg.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Borohydrides / pharmacokinetics
  • Boron / analysis
  • Boron / pharmacokinetics
  • Boron Compounds / pharmacokinetics
  • Boron Neutron Capture Therapy*
  • Drug Carriers
  • Female
  • Hindlimb
  • Hydrophobic and Hydrophilic Interactions
  • Injections, Intraperitoneal
  • Mammary Neoplasms, Experimental / radiotherapy*
  • Mice
  • Mice, Inbred BALB C
  • Phenylalanine / analogs & derivatives*
  • Phenylalanine / pharmacokinetics
  • Sulfhydryl Compounds / pharmacokinetics
  • Thorax
  • Tissue Distribution

Substances

  • Borohydrides
  • Boron Compounds
  • Drug Carriers
  • Sulfhydryl Compounds
  • mercaptoundecahydrododecaborate
  • Phenylalanine
  • Boron
  • 4-boronophenylalanine