beta-Conglycinin and glycinin in high-protein soybean seeds

J Agric Food Chem. 2001 Feb;49(2):729-35. doi: 10.1021/jf001110s.


The agronomic performance and storage proteins of high seed protein lines of soybeans [Glycine max L. (Merr.)] were investigated to determine if the two major storage proteins, beta-conglycinin and glycinin, contribute to the increased protein content of high seed protein lines. Subunits of these two major storage proteins were estimated by scanning SDS-PAGE gels by scanning densitometry. The relative rankings of the lines with respect to seed size and protein content were not different between years in one environment over 5 years, but oil and total protein and oil contents and the ratio of protein to oil differed. The alpha', alpha, and beta subunits of beta-conglycinin were significantly higher in the high-protein lines except CX797-115, CX804-108, CX804-3, D81-8498, and NC-2-62. The acidic A(3) polypeptide of glycinin was significantly higher in high-protein lines except 76-48773, CX804-108, CX804-3, D81-8498, and NC-2-62, whereas the acidic polypeptides A(1,2,4) of glycinin were significantly higher in all of the high-protein lines. The basic polypeptides of glycinin were significantly higher in all high seed protein lines except D81-8259. In conclusion, high-protein lines appear to contain more beta-conglycinin and glycinin than normal-protein soybean lines, and the amounts of subunits and polypeptides differ among lines.

Publication types

  • Comparative Study

MeSH terms

  • Antigens, Plant
  • Electrophoresis, Polyacrylamide Gel / methods
  • Globulins / analysis*
  • Plant Proteins / analysis
  • Protein Subunits
  • Seed Storage Proteins
  • Seeds / chemistry*
  • Soybean Oil / chemistry
  • Soybean Proteins*
  • Soybeans / chemistry*
  • Species Specificity


  • Antigens, Plant
  • Globulins
  • Plant Proteins
  • Protein Subunits
  • Seed Storage Proteins
  • Soybean Proteins
  • beta-conglycinin protein, Glycine max
  • Soybean Oil
  • glycinin