Limiting DNA Replication to Once and Only Once

EMBO Rep. 2000 Dec;1(6):479-83. doi: 10.1093/embo-reports/kvd116.


In Escherichia coli cells, the origin of chromosomal replication is temporarily inactivated after initiation has occurred. Origin sequestration is the first line of defence against over-initiation, providing a time window during which the initiation potential can be reduced by: (i) titration of DnaA proteins to newly replicated chromosomal elements; (ii) regulation of the activity of the DnaA initiator protein; and (iii) sequestration of the dnaA gene promoter. This review represents the first attempt to consider together older and more recent data on such inactivation mechanisms in order to analyze their contributions to the overall tight replication control observed in vivo. All cells have developed mechanisms for origin inactivation, but those of other bacteria and eukaryotic cells are clearly distinct from those of E. coli. Possible differences and similarities are discussed.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Bacterial Proteins / genetics
  • Bacterial Proteins / physiology
  • Chromosomes / metabolism
  • DNA Methylation
  • DNA Replication*
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / physiology
  • Escherichia coli / genetics*
  • Escherichia coli / metabolism*
  • Eukaryotic Cells / metabolism
  • Models, Genetic
  • Promoter Regions, Genetic


  • Bacterial Proteins
  • DNA-Binding Proteins
  • DnaA protein, Bacteria