Development of the tight-skin phenotype in immune-deficient mice

Arthritis Rheum. 2001 Mar;44(3):723-7. doi: 10.1002/1529-0131(200103)44:3<723::AID-ANR122>3.0.CO;2-V.


Objective: To determine if cutaneous thickening, a major phenotypic feature of the tight-skin (Tsk) mutation, could develop in an immune-deficient mouse.

Methods: Experimental crosses among different strains of mice were conducted to create mice that were genetically Tsk/+, and that were also homozgyous for a mutation at the Prkdc(scid) locus and thus lacked mature T and B lymphocytes. Skin samples prepared from experimental and control genotypic groups of mice were evaluated for skin thickness.

Results: The data showed that the Tsk/+ mice developed the Tsk phenotype in the absence of a functional immune system.

Conclusion: Mature T and B cells are not required for the development of the cutaneous thickening in mice carrying the Tsk mutation.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Acquired Immunodeficiency Syndrome / genetics*
  • Animals
  • Disease Models, Animal
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Mice, Transgenic
  • Phenotype
  • Scleroderma, Systemic / genetics*
  • Scleroderma, Systemic / immunology*