MPtopo: A database of membrane protein topology

Protein Sci. 2001 Feb;10(2):455-8. doi: 10.1110/ps.43501.


The reliability of the transmembrane (TM) sequence assignments for membrane proteins (MPs) in standard sequence databases is uncertain because the vast majority are based on hydropathy plots. A database of MPs with dependable assignments is necessary for developing new computational tools for the prediction of MP structure. We have therefore created MPtopo, a database of MPs whose topologies have been verified experimentally by means of crystallography, gene fusion, and other methods. Tests using MPtopo strongly validated four existing MP topology-prediction algorithms. MPtopo is freely available over the internet and can be queried by means of an SQL-based search engine.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Algorithms
  • Cell Membrane / chemistry*
  • Databases, Factual*
  • Internet
  • Proteins / chemistry*
  • Software


  • Proteins