COS-l, a putative two-component histidine kinase of Candida albicans, is an in vivo virulence factor

Med Mycol. 2001 Feb;39(1):69-74. doi: 10.1080/mmy.39.1.69.74.

Abstract

The human fungal pathogen, Candida albicans, has three putative histidine kinases showing homology to those of plants, bacteria and other fungi. We have constructed a homozygous deletion strain and a hemizygous reconstituted strain of one of these histidine-kinase-encoding genes, COS-1, in C. albicans. Neither strain showed any growth defect in a number of liquid media nor increased resistance or sensitivity to a number of antifungal drugs. Importantly, we show that the COS-1 homozygous disruption strain had significantly reduced virulence in a systemic murine model of candidosis. Thus, COS-1 appears to be an in vivo virulence factor and may represent a novel target for the development of antifungal drugs.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Candida albicans / enzymology*
  • Candida albicans / genetics
  • Candida albicans / pathogenicity
  • Candidiasis / microbiology
  • Disease Models, Animal
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism
  • Genes, Fungal
  • Histidine Kinase
  • Humans
  • Mice
  • Protein Kinases / genetics*
  • Protein Kinases / physiology
  • Signal Transduction
  • Virulence / genetics

Substances

  • Fungal Proteins
  • Protein Kinases
  • Histidine Kinase