Tyrosine kinase participates in alpha 1A-adrenoceptor-mediated increase of intracellular calcium in human embryo kidney 293 cells

Zhongguo Yao Li Xue Bao. 1999 Nov;20(11):1025-30.

Abstract

Aim: To determine the role of protein-tyrosine kinase (PTK) in alpha 1A-adrenoceptor-mediated increase of [Ca2+]i (intracellular calcium) in human embryo kidney (HEK) 293 cells expressed alpha 1A-adrenoceptor.

Methods: Effects of two PTK inhibitors: genistein and tyrphostin, were investigated on the increase of [Ca2+]i by using Fura-2, The activity of PTK was measured and the accumulation of [3H] InsPs were observed.

Results: Norepinephrine stimulated a rapid increase in [Ca2+]i to (371 +/- 31) nmol.L-1 in HEK 293 cells. Norepinephrine-induced increase of [Ca2+]i was inhibited by the tyrosine kinase inhibitors quercetin and tyrphostin by 23.8% and 21.4%, respectively, but the accumulation of [3H]InsPs induced by norepinephrine was not. The activity of the plasma-associated tyrosine kinase was increased to (1.73 +/- 0.72)-fold over the control by norepinephrine 10 mumol.L-1. The norepinephrine-activated PTK was inhibited by calphostin C and depletion of intra- and extra-cellular Ca2+.

Conclusion: The PTK participates in mobilization of Ca2+ mediated by alpha 1A-adrenoceptors in HEK 293 cell lines.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Calcium / metabolism*
  • Cell Line
  • Embryo, Mammalian
  • Genistein / pharmacology
  • Humans
  • Kidney / cytology
  • Kidney / metabolism*
  • Naphthalenes / pharmacology
  • Norepinephrine / pharmacology
  • Phentolamine / pharmacology
  • Protein-Tyrosine Kinases / antagonists & inhibitors*
  • Protein-Tyrosine Kinases / metabolism
  • Receptors, Adrenergic, alpha-1 / physiology*
  • Tyrphostins / pharmacology

Substances

  • ADRA1A protein, human
  • Naphthalenes
  • Receptors, Adrenergic, alpha-1
  • Tyrphostins
  • Genistein
  • Protein-Tyrosine Kinases
  • calphostin C
  • Calcium
  • Norepinephrine
  • Phentolamine