glnD and mviN are genes of an essential operon in Sinorhizobium meliloti

J Bacteriol. 2001 Apr;183(8):2682-5. doi: 10.1128/JB.183.8.2682-2685.2001.

Abstract

To evaluate the role of uridylyl-transferase, the Sinorhizobium meliloti glnD gene was isolated by heterologous complementation in Azotobacter vinelandii. The glnD gene is cotranscribed with a gene homologous to Salmonella mviN. glnD1::Omega or mviN1::Omega mutants could not be isolated by a powerful sucrose counterselection procedure unless a complementing cosmid was provided, indicating that glnD and mviN are members of an indispensable operon in S. meliloti.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / genetics*
  • Bacterial Proteins / metabolism
  • Cloning, Molecular
  • Conjugation, Genetic
  • Genes, Bacterial
  • Genes, Essential
  • Genetic Complementation Test
  • Molecular Sequence Data
  • Mutagenesis, Insertional
  • Nucleotidyltransferases / genetics*
  • Nucleotidyltransferases / metabolism
  • Operon*
  • PII Nitrogen Regulatory Proteins
  • Plasmids
  • Polymerase Chain Reaction
  • Sequence Analysis, DNA
  • Sinorhizobium meliloti / genetics
  • Sinorhizobium meliloti / metabolism*

Substances

  • Bacterial Proteins
  • PII Nitrogen Regulatory Proteins
  • Nucleotidyltransferases
  • regulatory protein uridylyltransferase

Associated data

  • GENBANK/AF227730