Determination of 24(R)-pseudoginsenoside F(11) in North American ginseng using high performance liquid chromatography with evaporative light scattering detection

W Li; J F Fitzloff

doi:10.1016/s0731-7085(00)00494-5

Determination of 24(R)-pseudoginsenoside F(11) in North American ginseng using high performance liquid chromatography with evaporative light scattering detection

J Pharm Biomed Anal. 2001 May;25(2):257-65. doi: 10.1016/s0731-7085(00)00494-5.

Authors

W Li¹, J F Fitzloff

Affiliation

¹ Functional Foods for Health (FFH) Core Analytical Laboratory, Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Illinois at Chicago, 833 South Wood Street, Chicago, IL 60612, USA.

PMID: 11275434
DOI: 10.1016/s0731-7085(00)00494-5

Abstract

A gradient liquid chromatographic method with evaporative light scattering detection (ELSD) for the determination of 24(R)-pseudoginsenoside F(11) in North American ginseng is described. Samples are analyzed by means of a reverse-phase column (Waters Spherisorb ODS-2, C(18)) using acetonitrile and water under gradient conditions as the mobile phase over 20 min. The evaporative light scattering detector (ELSD) used, was set at an evaporating temperature of 35 degrees C and nitrogen gas pressure of 3.4 bar. The detection limit (S/N>5) of 24(R)-pseudoginsenoside F(11) is 53 ng on column.

MeSH terms

Calibration
Chromatography, High Pressure Liquid
Ginsenosides*
Indicators and Reagents
Light
Panax / chemistry*
Plants, Medicinal*
Reference Standards
Reproducibility of Results
Saponins / analysis*
Scattering, Radiation
Spectrophotometry, Ultraviolet

Substances

Ginsenosides
Indicators and Reagents
Saponins
pseudoginsenoside F11