Determination of protein-protein interactions of ICIn by the yeast two-hybrid system

Cell Physiol Biochem. 2001;11(1):55-60. doi: 10.1159/000047792.

Abstract

ICln is a ubiquitously expressed eukaryotic protein. Expression of the protein in Xenopus laevis oocytes, the knocking-down of the protein in fibroblasts, or the reconstitution of the protein in lipid bilayer led to the assumption that this protein is an ionic channel or a significant part thereof. However, other possible roles for ICln in potential regulatory mechanisms have been postulated, as diverse as regulator of cell morphology by interacting with the Skb1 protein and/or interaction with core spliceosomal proteins. Here we show that ICln is able to interact with SnRNP core proteins SmD1, SmD2, SmD3, SmX5 and SmB/B'.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Gene Library
  • Genes, Reporter / genetics
  • Humans
  • Ion Channels*
  • Molecular Sequence Data
  • Protein Binding
  • Proteins / genetics*
  • Proteins / metabolism*
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism*
  • Ribonucleoproteins, Small Nuclear / chemistry
  • Ribonucleoproteins, Small Nuclear / genetics
  • Ribonucleoproteins, Small Nuclear / metabolism*
  • Saccharomyces cerevisiae / genetics
  • Two-Hybrid System Techniques*

Substances

  • CLNS1A protein, human
  • Ion Channels
  • Lsm2 protein, mouse
  • Proteins
  • Recombinant Fusion Proteins
  • Ribonucleoproteins, Small Nuclear

Associated data

  • GENBANK/AF196468