EUS-guided fine-needle aspiration combined with flow cytometry and immunocytochemistry in the diagnosis of lymphoma

Gastrointest Endosc. 2001 Apr;53(4):485-91. doi: 10.1067/mge.2001.112841.


Background: Limited information is available regarding the use of EUS-guided fine-needle aspiration (EUS-FNA) in the diagnosis of lymphoproliferative disorders. The aim of this study was to evaluate the yield of this technique in the primary diagnosis of lymphoma.

Methods: The records were reviewed of 38 consecutive patients with GI lesions and/or enlarged lymph nodes identified on imaging studies that raised a suspicion of lymphoma who underwent EUS-FNA of lymph nodes or the gut wall. Final diagnosis was based on clinical follow-up, imaging studies, or surgical findings.

Results: Twenty-three patients with lymphoma and 15 patients with benign disease or reactive lymphadenopathy were identified. The overall sensitivity, specificity, and accuracy of EUS-FNA cytology with flow cytometry/immunocytochemistry (FC/IC) for the diagnosis of lymphoma were, respectively, 74%, 93%, and 81%. When comparing patients who had EUS-FNA with FC/IC versus those who had EUS-FNA without FC/IC, sensitivity was 86% versus 44% (p = 0.04), specificity was 100% versus 90% (not significant), and accuracy was 89% versus 68% (not significant).

Conclusion: EUS-FNA can provide cytology specimens diagnostic for lymphoma. Selective use of FC/IC in patients with suspected lymphoma improves the yield of EUS-FNA and may guide diagnostic evaluation and treatment decisions.

Publication types

  • Evaluation Study

MeSH terms

  • Adult
  • Aged
  • Biopsy, Needle / methods*
  • Endoscopy, Digestive System / methods*
  • Female
  • Flow Cytometry
  • Humans
  • Immunohistochemistry
  • Lymph Node Excision
  • Lymph Nodes / diagnostic imaging
  • Lymphatic Diseases / diagnosis
  • Lymphoma / classification
  • Lymphoma / diagnosis*
  • Lymphoma / diagnostic imaging
  • Lymphoma / pathology
  • Male
  • Middle Aged
  • Retrospective Studies
  • Sensitivity and Specificity
  • Ultrasonography