Porcine HveC, a member of the highly conserved HveC/nectin 1 family, is a functional alphaherpesvirus receptor

Virology. 2001 Mar 15;281(2):315-28. doi: 10.1006/viro.2000.0798.


Human herpesvirus entry mediator C (HveC) is an alphaherpesvirus receptor which binds to virion glycoprotein D (gD). We identified porcine HveC and studied its interaction with pseudorabies virus (PrV) and herpes simplex virus type 1 (HSV-1) gD. Porcine and human HveC have 96% amino acid identity and HveC from African green monkey, mouse, hamster, and cow are similarly conserved. Porcine HveC mediates entry of HSV-1, HSV-2, PrV, and bovine herpesvirus type 1. Truncated soluble forms of HSV-1 and PrV gD bind competitively to porcine HveC. Biosensor analysis shows that PrV gD binds with a 10-fold higher affinity than HSV-1 gD. Monoclonal antibodies against human HveC recognize the porcine homologue and can block gD binding and entry of HSV-1 and PrV. Porcine HveC is functionally indistinguishable from human HveC. Our results are consistent with the suggestion that HveC is a pan-alphaherpesvirus receptor that interacts with a conserved structural domain of gD.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alphaherpesvirinae / physiology*
  • Amino Acid Sequence
  • Animals
  • Antibodies / immunology
  • Binding, Competitive
  • Cell Adhesion Molecules / genetics
  • Cell Adhesion Molecules / immunology
  • Cell Adhesion Molecules / metabolism*
  • Cell Line
  • Chlorocebus aethiops
  • Cloning, Molecular
  • Herpesvirus 1, Suid / physiology
  • Humans
  • Molecular Sequence Data
  • Nectins
  • Protein Binding
  • Receptors, Virus / genetics
  • Receptors, Virus / metabolism*
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Swine
  • Vero Cells
  • Viral Envelope Proteins / metabolism


  • Antibodies
  • Cell Adhesion Molecules
  • NECTIN1 protein, human
  • Nectin1 protein, mouse
  • Nectins
  • Receptors, Virus
  • Viral Envelope Proteins
  • glycoprotein D, Human herpesvirus 1