A novel group of phospholipase A2s preferentially expressed in type 2 helper T cells

J Biol Chem. 2001 May 25;276(21):18321-6. doi: 10.1074/jbc.M008837200. Epub 2001 Mar 12.


We report a novel phospholipase A(2) (PLA(2)), group XII (GXII) PLA(2), distinct from other cysteine-rich groups with a catalytic histidine motif, by its 20-kDa size and distribution of the 14 cysteine residues within the protein. Alternative spliced forms with distinct subcellular localization, designated GXII-1 and GXII-2, were identified by reverse transcription-polymerase chain reaction. Importantly, GXII PLA(2)s, in particular GXII-2 PLA(2), and group V PLA(2), but not group X PLA(2), were selectively expressed in murine type 2 helper T (Th2) clones and in vitro differentiated mouse CD4 Th2 cells as compared with type 1 helper T clones and cells. Stimulation with anti-CD3 appreciably up-regulated expression of GXII PLA(2)s and group V PLA(2) by steady state analysis of the Th2 cells as compared with type 1 helper T cells. These results suggest that group XII and group V PLA(2)s might participate in helper T cell immune response through release of immediate second signals and generation of downstream eicosanoids.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alternative Splicing
  • Amino Acid Sequence
  • Animals
  • Cell Line
  • Cloning, Molecular
  • Molecular Sequence Data
  • Phospholipases A / genetics*
  • Phospholipases A / immunology
  • Phospholipases A / metabolism
  • Sequence Alignment
  • Sequence Analysis
  • Th2 Cells / enzymology*
  • Th2 Cells / immunology


  • Phospholipases A

Associated data

  • GENBANK/AY007381
  • GENBANK/AY007382