A family of 16-kDa pancreatic secretory stress proteins form highly organized fibrillar structures upon tryptic activation

J Biol Chem. 2001 Jun 15;276(24):21028-38. doi: 10.1074/jbc.M010717200. Epub 2001 Mar 16.

Abstract

A group of 16-kDa proteins, synthesized and secreted by rat pancreatic acinar cells and composed of pancreatic stone protein (PSP/reg) and isoforms of pancreatitis-associated protein (PAP), show structural homologies, including conserved amino acid sequences, cysteine residues, and highly sensitive N-terminal trypsin cleavage sites, as well as conserved functional responses in conditions of pancreatic stress. Trypsin activation of recombinant stress proteins or counterparts contained in rat pancreatic juice (PSP/reg, PAP I and PAP III) resulted in conversion of 16-kDa soluble proteins into 14-kDa soluble isoforms (pancreatic thread protein and pancreatitis-associated thread protein, respectively) that rapidly polymerize into insoluble sedimenting structures. Activated thread proteins show long lived resistance to a wide spectrum of proteases contained in pancreatic juice, including serine proteases and metalloproteinases. In contrast, PAP II, following activation with trypsin or pancreatic juice, does not form insoluble structures and is rapidly digested by pancreatic proteases. Scanning and transmission electron microscopy indicate that activated thread proteins polymerize into highly organized fibrillar structures with helical configurations. Through bundling, branching, and extension processes, these fibrillar structures form dense matrices that span large topological surfaces. These findings suggest that PSP/reg and PAP I and III isoforms consist of a family of highly regulated soluble secretory stress proteins, which, upon trypsin activation, convert into a family of insoluble helical thread proteins. Dense extracellular matrices, composed of helical thread proteins organized into higher ordered matrix structures, may serve physiological functions within luminal compartments in the exocrine pancreas.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Calcium-Binding Proteins / chemistry
  • Calcium-Binding Proteins / metabolism*
  • Calcium-Binding Proteins / ultrastructure
  • Cloning, Molecular
  • Genetic Vectors
  • Heat-Shock Proteins / chemistry
  • Heat-Shock Proteins / metabolism*
  • Heat-Shock Proteins / ultrastructure
  • Kinetics
  • Lithostathine
  • Microscopy, Electron
  • Molecular Sequence Data
  • Nerve Tissue Proteins*
  • Pancreas / metabolism*
  • Pancreatitis-Associated Proteins
  • Peptide Fragments / chemistry
  • Phosphoproteins / chemistry
  • Phosphoproteins / metabolism
  • Phosphoproteins / ultrastructure
  • Pichia
  • Protein Isoforms / chemistry
  • Protein Isoforms / metabolism
  • Protein Isoforms / ultrastructure
  • Rats
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / metabolism
  • Recombinant Proteins / ultrastructure
  • Sequence Alignment
  • Trypsin / metabolism*

Substances

  • Calcium-Binding Proteins
  • Heat-Shock Proteins
  • Lithostathine
  • Nerve Tissue Proteins
  • Pancreatitis-Associated Proteins
  • Peptide Fragments
  • Phosphoproteins
  • Protein Isoforms
  • REG3A protein, human
  • Recombinant Proteins
  • Reg1a protein, rat
  • Trypsin