Effect of 1alpha-hydroxyvitamin D3 and egg-shell calcium on bone metabolism in ovariectomized osteoporotic model rats

J Bone Miner Metab. 2001;19(2):84-8. doi: 10.1007/s007740170045.


Egg-shell calcium (Ca) is one of the effective Ca sources for bone metabolism. In the present study, we investigated whether egg-shell Ca had similar effects compared with calcium carbonate (CaCO3) when vitamin D3 (1alpha(OH)D3) treatment was given to an osteoporotic rat model. In both 1alpha(OH)D3-supplemented and -unsupplemented rats, the bone mineral density (BMD) of the lumber spine in the vitamin-supplemented group increased significantly compared with the unsupplemented group. In a Ca balance study, there were also significant differences in intestinal Ca absorption, urinary Ca and fecal Ca between the vitamin-supplemented and -unsupplemented groups. These results show that egg-shell Ca could have similar effects to CaCO3 on bone metabolism. In contrast with CaCO3, vitamin D3 supplementation did not significantly increase serum Ca levels in the egg-shell Ca group; however, the mechanism of Ca absorption is still unclear. Our results suggest that egg-shell Ca may be an effective nutrient in Ca metabolism for people treated with vitamin D3.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bone Density
  • Bone and Bones / metabolism*
  • Calcium / metabolism*
  • Dietary Supplements
  • Disease Models, Animal
  • Female
  • Femur / metabolism
  • Hydroxycholecalciferols / metabolism*
  • Lumbar Vertebrae / metabolism
  • Osteoporosis / metabolism*
  • Ovariectomy
  • Rats
  • Tibia / metabolism


  • Hydroxycholecalciferols
  • Calcium
  • alfacalcidol