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, 72 (4), 603-13

Nutrient Preference and Diet-Induced Adiposity in C57BL/6ByJ and 129P3/J Mice

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Nutrient Preference and Diet-Induced Adiposity in C57BL/6ByJ and 129P3/J Mice

A A Bachmanov et al. Physiol Behav.

Abstract

Purified carbohydrates and fats are usually palatable to humans and other animals, and their consumption often induces weight gain and accumulation of fat. In this study, we examined consumption of complex carbohydrates (cornstarch and Polycose) and fats (soybean oil and margarine) in mice from two inbred strains, C57BL/6ByJ and 129P3/J. At lower concentrations of liquid nutrients tested using two-bottle tests, when the amounts consumed had negligible energy content, the C57BL/6ByJ mice had higher acceptance of Polycose and soybean oil. This was probably due to strain differences in chemosensory perception of Polycose and oil. At higher concentrations, the mice consumed a substantial part of their daily energy from the macronutrient sources, however, there were no or only small strain differences in nutrient consumption. These small differences were probably due to strain variation in body size. The two strains also did not differ in chow intake. Despite similar energy intakes, access to the nutrients resulted in greater body weight (BW) gain in the C57BL/6ByJ mice than in the 129P3/J mice. The diet-induced weight gain was examined in detail in groups of 2-month-old C57BL/6ByJ and 129P3/J mice given ether chow, or chow and margarine to eat. Access to margarine did not increase total energy consumption of either strain. It increased BW and adiposity of the C57BL/6ByJ mice, but only after they reached the age of approximately 3 months. There were no differences in BW and adiposity between control and margarine-exposed 129P3/J mice. The results suggest that diet-induced adiposity in the B6 mice depends on age and does not depend on hyperphagia.

Figures

Fig. 1
Fig. 1
Average daily intakes of nutrient emulsions or solutions (the top two rows), energy derived from the nutrients (the third and fourth rows), and nutrient preferences (the bottom row) by B6 and 129 mice in two-bottle 48-h preference tests. (A) Cornstarch emulsions (Experiment 2); (B) Polycose solutions (Experiment 3); (C) Soybean oil emulsions (Experiment 4). Vertical bars represent S.E.M. * Significant difference between B6 and 129 mice; P < .05, post hoc tests, ANOVA with initial BW as a covariate (intakes per mouse), or with no covariates (intakes per BW and preferences).
Fig. 2
Fig. 2
Average daily intakes of margarine and energy derived from margarine by B6 and 129 mice in Experiment 2. Vertical bars represent S.E.M. * Significant difference between B6 and 129 mice; P < .05, ANOVA with initial BW as a covariate (intakes per mouse), or t tests (intakes per BW).
Fig. 3
Fig. 3
BW changes of B6 and 129 mice during access to nutrients in Experiments 2, 3, and 4. Vertical bars represent S.E.M. Initial and final BWs were measured before and after tests with a nutrient, respectively. Significant differences are indicated by * (B6 vs. 129 strains) or # (initial vs. final BW); P < .05; post hoc tests, ANOVA with no covariates (Table 2).
Fig. 4
Fig. 4
Average daily intakes of margarine (A), chow (B), and energy derived from all food sources (C), and BW (D) of B6 and 129 mice in Experiment 5. Vertical bars represent S.E.M. On Week 0, all mice had only chow to eat. On Weeks 1–10, margarine groups had margarine in addition to chow. Significant differences between control and margarine-exposed mice are indicated by * (B6 mice) or + (129 mice); P < .05; post hoc tests, ANOVA with BW before access to margarine (Week 0) as a covariate (A–C) or with no covariates (D).

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