Increase of free oxygen radicals in aqueous humour induced by selective Nd:YAG laser trabeculoplasty in the rabbit

Eur J Ophthalmol. 2001 Jan-Mar;11(1):47-52. doi: 10.1177/112067210101100109.


Purpose: To investigate the impact of selective Nd:YAG laser trabeculoplasty on free oxygen radicals and antioxidant enzymes of the aqueous humour in the rabbit.

Methods: One eye of 18 rabbits was subjected to 360 degrees selective laser trabeculoplasty (LT) with a frequency-doubled Nd:YAG laser (532 nm). The anterior chamber aqueous humour was aspirated 3, 12 hours and 1, 3, 7, 10 days after treatment. Lipid peroxide (LPO) and glutathione S transferase (GST) levels and superoxide dismutase (SOD) activities of aqueous humour were measured.

Results: Concentrations of LPO in the aqueous humour of the treated eyes were significantly higher than the untreated eyes until the 7th day. Aqueous SOD activity significantly decreased 3 hours after LT and remained low until day 7. Aqueous GST levels were significantly decreased between 12 hours and 7 days after the LT.

Conclusions: Selective LT was followed by an immediate increase in the aqueous humour LPO concentration and decreases of SOD and GST in the rabbit, probably due to photovaporization and photodisruption caused by the frequency-doubled Nd:YAG laser. The increased aqueous LPO levels suggest that free oxygen radicals are formed in the pigmented trabecular meshwork during LT, and may be responsible for the inflammatory complications of this procedure.

MeSH terms

  • Animals
  • Aqueous Humor / metabolism*
  • Free Radicals / metabolism
  • Glutathione Transferase / metabolism
  • Laser Therapy*
  • Lipid Peroxidation*
  • Lipid Peroxides / metabolism
  • Rabbits
  • Superoxide Dismutase / metabolism
  • Superoxides / metabolism*
  • Time Factors
  • Trabecular Meshwork / metabolism
  • Trabecular Meshwork / surgery
  • Trabeculectomy*


  • Free Radicals
  • Lipid Peroxides
  • Superoxides
  • Superoxide Dismutase
  • Glutathione Transferase